Metabolites were identified by matching the retention time and mass (±10 ppm) to authentic standards. Peak areas were integrated using Profinder v8.00 (Agilent Technologies, Santa Clara, California). Data were normalized to urine creatinine levels and Loess drift correction, using Metabodrift 1.0 (Thonusin et al., 2017 (link)) and applied using the area of each metabolite in the control samples (pools) for correction.
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