Evaluating L-norvaline Effects on Alzheimer's Pathology

Homozygous triple-transgenic mice (3×Tg-AD) harboring PS1(M146V), APP(Swe), and tau(P301L) transgenes were purchased from Jackson Laboratory (Bar Harbor, ME, USA) and bred in our animal facility. The 3×Tg-AD mice exhibited a synaptic dysfunction, plaque, and tangle pathology [84 (link)]. Four-month-old male 3×Tg-AD mice and age-matched male C57Bl/6 mice were divided randomly into four equal (20 mice each) groups according to their strain. The animals were housed in separate cages (five animals each) in constant ambient conditions and provided with water and food ad libitum. L-norvaline (Sigma) was dissolved in the animals’ drinking water (250 mg/L) and supplied in the animals’ cages for ten weeks in accordance with the previously published protocol [27 (link)]. The Bar-Ilan University animal care and use committee approved the experimental protocol (approval No. 82-10-2017) on October 1, 2017.

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Polis B., Gurevich V., Assa M, & Samson A.O. (2019). Norvaline Restores the BBB Integrity in a Mouse Model of Alzheimer’s Disease. International Journal of Molecular Sciences, 20(18), 4616.

Publication 2019

tau(P301L) L-norvaline

Animal C57bl mice Food Homozygous Male Mice Norvaline Plaque Strain Transgenes Transgenic mice

Corresponding Organization :

Other organizations : Bar-Ilan University

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Variable analysis

independent variables

Treatment with L-norvaline (250 mg/L in drinking water)

dependent variables

Synaptic dysfunction
Plaque pathology
Tangle pathology

control variables

Age (4-month-old)
Sex (male)
Strain (C57Bl/6 and 3×Tg-AD)
Housing conditions (separate cages, 5 animals each, constant ambient conditions)
Diet and water (ad libitum)

controls

Positive control: 3×Tg-AD mice (exhibiting synaptic dysfunction, plaque, and tangle pathology)
Negative control: Age-matched C57Bl/6 mice

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