Synthesis and Characterization of Chitosan Nanoparticles

Blank CSNPs were prepared by adding 1 mL of a 1% sodium tripolyphosphate solution dropwise to a chitosan solution under constant stirring over 3–4 hours. The blank nanoparticles were designated as BNP. Prior to any testing, the CSNP-containing solution was stirred with a magnetic stirrer (Corning PC-420D, Seoul, South Korea) for 15 min at room temperature to ensure the homogenous dispersion of nanoparticles. The drops of nanoparticles spread uniformly on the glass slide and were left to dry [27 (link)]. The dried nanoparticles were inspected with a scanning electron microscope (JEOL JSM-6390 LA, Tokyo, Japan) at a resolution of 10kV and 5,000–50,000 x.