WI-38 cells on poly-lysine coated chamber slides (BD Biosciences) were mock infected or infected with RV strains at MOI = 5 (two experiments with two replicate wells per each virus strain). RV genomic RNA was detected with the positive strand-specific probe set using the QuantiGene ViewRNA assay kit (Affymetrix, Cat # QV0096) as previously described [21 (link)].
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