Western Blotting Quantification and Antibody Detection

Western blotting was performed as previously (18 , 19 (link)) and visualized and quantified using a Fluor-S Multi-Imager with Multi-Analyst software (Bio-Rad, Hercules, CA, USA). We utilized a rabbit anti-Ctsk antibody previously generated in our lab (11 (link)) and a goat anti-rabbit IgG-HRP (7074S, Cell signaling, Danvers, MA, USA) for this Western analysis.

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Chen W., Gao B., Hao L., Zhu G., Jules J., Macdougall M.J., Han X., Zhou X, & Li Y.P. (2016). Gene Therapy of Periodontal Disease by AAV-shRNA Silencing of Cathepsin K by inhibiting inflammation in periodontal lesion area. Journal of periodontal research, 51(5), 647-660.

Publication 2016

Fluor-S Multi-Imager Multi-Analyst goat anti-rabbit IgG-HRP

Anti antibody Anti igg Ctsk Goat Rabbit

Corresponding Organization : In-Q-Tel

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Variable analysis

independent variables

Western blotting

dependent variables

Ctsk protein expression
Protein quantification

control variables

Western blotting protocol as previously (18 (no link found), 19 (link))
Fluor-S Multi-Imager with Multi-Analyst software (Bio-Rad, Hercules, CA, USA)
Rabbit anti-Ctsk antibody previously generated in our lab (11 (link))
Goat anti-rabbit IgG-HRP (7074S, Cell signaling, Danvers, MA, USA)

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