Lyophilized α-syn was dissolved in sterile PBS and filtered through a 0.2 μm filter to remove any pre-formed aggregates. α-syn fibrils were generated by incubating purified α-syn at 5 mg/mL at 37 °C under constant agitation at 1000 rpm in a Thermomix R shaker (Eppendorf, Hamburg, Germany) for a period of 5 days. The fibrils generated were then aliquoted and stored at −80 °C until use50 (link).
Free full text: Click here