Antibody and Inhibitor Procurement for Signaling Pathway Analysis

Antibody against ERα was obtained from Santa Cruz Biotechnology. ERβ–MC-10 antibody was developed and characterized by Dr. Hawse’s laboratory (Wu et al., 2012 (link)). Fluorescent secondary antibodies were obtained from Li-Cor Biosciences (Lincoln, NE). Pharmacological inhibitors for the signaling pathways NFκB (SN50), P42/44 (PD98059), PI3K (Wortmannin) and P38 (SB203580) were obtained from EMD/Calbiochem (Culver City, CA), STAT6 (AS1517499) from Axon MedChem (Reston, VA), and AP1 (SR11302) from Tocris (Minneapolis, MN). TNFα and IL-13 were obtained from R&D systems (Minneapolis, MN). All other chemicals or antibodies were obtained from Sigma-Aldrich.

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Aravamudan B., Goorhouse K.J., Unnikrishnan G., Thompson M.A., Pabelick C.M., Hawse J.R., Prakash Y, & Sathish V. (2017). Differential Expression of Estrogen Receptor Variants in Response to Inflammation Signals in Human Airway Smooth Muscle. Journal of cellular physiology, 232(7), 1754-1760.

Publication 2017

Antibody against ERα Fluorescent secondary antibodies TNFα IL-13

Antibodies Antibody As1517499 Axon Fluorescent antibodies Il 13 Nfκb inhibitors Pd98059 Pi3k Reston Sb203580 Signaling pathways Sr11302 Stat6 Tnfα Wortmannin

Corresponding Organization : North Dakota State University

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Variable analysis

independent variables

NFκB (SN50)
P42/44 (PD98059)
PI3K (Wortmannin)
P38 (SB203580)
STAT6 (AS1517499)
AP1 (SR11302)
TNFα
IL-13

dependent variables

Not explicitly mentioned

control variables

Antibody against ERα from Santa Cruz Biotechnology
ERβ–MC-10 antibody developed and characterized by Dr. Hawse's laboratory
Fluorescent secondary antibodies from Li-Cor Biosciences

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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