Murine Cell Lines for Tumor Research

CMS5a is a 3-methylcholanthrene-induced fibrosarcoma cell line of BALB/c origin. CMS5a-NY-ESO-1 is a cell line derived from CMS5a stably transfected with New York esophageal squamous cell carcinoma 1 (NY-ESO-1).22 (link) B16F0 is a naturally developed malignant melanoma cell line in C57BL/6 mice. CMS5a, CMS5a-NY-ESO-1 and B16F0 were maintained in RPMI-1640 supplemented with 10% fetal bovine serum and 4 mmol/L of L-glutamine (CSTI, Miyagi, Japan) and were used after confirming that they were negative for Mycoplasma, as determined by testing with a PCR Mycoplasma Detection Kit (TaKaRa) according to the manufacturer’s instructions. Female BALB/c and C57BL/6 mice aged 6–7 weeks old were purchased from CLEA Japan (Tokyo, Japan). All mice were maintained in a specific pathogen-free facility at National Cancer Center Japan (Tokyo/Chiba, Japan).