For worm samples, proteins were extracted as described previously13 (link). Western blotting was performed with antibodies against GFP (1:1000, Cat. 2956, CST), Actin (1:2000, Cat. A5441, Sigma), H3K18Ac (1:1000, Cat. 07-354, Merck), H3K27Ac (1:1000, Cat. ab4729, abcam), H3K9Ac (1:1000, Cat. 06-942, Merck), H3K4Ac (1:1000, Cat. Ab176799, abcam), Histone 3 (1:1000, Cat. 9715, CST), Tubulin (1:2000, Cat. T5168, Sigma), H3K27Me3 (1:1000, Cat. 07-449, millipore), H3K27Me2 (1:1000, Cat. ab24684, abcam), H3K27Me1 (1:1000, Cat. 07-448, millipore), H3K9Me1 (1:1000, Cat. 07-450, millipore), H3K4Me3 (1:1000, Cat. 07-473, millipore), Histone 4 (1:1000, Cat. sc-10810, Santa Cruz), H4K5Ac (1:1000, Cat. ab51997, abcam), β-amyloid 1–16 (6E10) (1:1000, Cat. 803001, BioLegend), HA-tag (1:2000, Cat. 3724, CST), Flag-tag (1:1000, Cat. F7425, Sigma), Myc-tag (1:2000, Cat. sc-40, Santa Cruz), GST-tag (1:1000, Cat. 2625, CST), AcK (1:1000, Cat. 9441, CST), AcK (1:1000, Cat. 9814, CST) and HRP-labelled anti-rabbit (Cat. 7074, CST) and anti-mouse (Cat. 7076, CST) secondary antibodies.
Protocol detail
Protein Extraction and Western Blotting
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Corresponding Organization :
Other organizations : École Polytechnique Fédérale de Lausanne
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Variable analysis
independent variables
- Protein extraction method
- Protein levels of GFP
- Protein levels of Actin
- Protein levels of H3K18Ac
- Protein levels of H3K27Ac
- Protein levels of H3K9Ac
- Protein levels of H3K4Ac
- Protein levels of Histone 3
- Protein levels of Tubulin
- Protein levels of H3K27Me3
- Protein levels of H3K27Me2
- Protein levels of H3K27Me1
- Protein levels of H3K9Me1
- Protein levels of H3K4Me3
- Protein levels of Histone 4
- Protein levels of H4K5Ac
- Protein levels of β-amyloid 1–16 (6E10)
- Protein levels of HA-tag
- Protein levels of Flag-tag
- Protein levels of Myc-tag
- Protein levels of GST-tag
- Protein levels of AcK
- Worm samples
- Antibody dilutions
- None specified
- None specified
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