Rabbit and rat polyclonal anti-EHBP1L1 antibodies were raised as previously described (11 (link)). The following additional primary antibodies were used: rabbit anti-pericentrin (ATLAS antibodies, #HPA016820); mouse anti-acetylated tubulin and anti-FLAG M2 (Sigma-Aldrich, #T7451 and #F1804); mouse anti-Arl13b (Abcam, #ab136648); rabbit anti-ß-actin, anti-Arl13b, anti-CD2AP, and mouse anti-GAPDH (Proteintech, #20536-1-AP, #17711-1-AP, #51046-1-AP, and #66004-1-Ig); mouse anti-CIN85 and anti-Arp2 (Santa Cruz Biotechnology, #sc-166862 and #sc-166103); rabbit anti-myosin Va (Cell signaling, #3402) and mouse anti-MBP (Developmental Studies Hybridoma Bank). The secondary antibodies Alexa Fluor 488 donkey anti-rabbit IgG, anti-mouse IgG, Alexa Fluor 568 donkey anti-rabbit IgG, and anti-mouse IgG were purchased from Thermo Fisher Scientific. Horseradish peroxidase–conjugated anti-rabbit IgG and anti-mouse IgG were purchased from Cell Signaling Technology.
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