Slides were analyzed by a confocal laser scanning system (LSM 700) using Nikon E600 (Japan) fluorescence microscope and Plan Apo x 40 immersion oil objective. Fluorescent intensities were integrated with Image J software (Wayne Rasband, NIH, USA). Menders Overlap Coefficient (MOC) was used to quantify co-localization (17 ). Histological observations were recorded by an observer who was blinded to the clinical information.
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