Endotoxin Shock Model in Mice

Endotoxin shock model was induced as previously described ^{7 (link),9 (link)}. Mice were administered with lipopolysaccharide (15 mg/kg body weight) (Escherichia coli 0111:B4, Sigma, St Louis, MO) by intra-peritoneal injection followed by administration of 1 ml of pre-warmed normal saline.

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Zhang M., Zou L., Feng Y., Chen Y.J., Zhou Q., Ichinose F, & Chao W. (2014). Toll-like receptor 4 is essential to preserving cardiac function and survival in low grade polymicrobial sepsis. Anesthesiology, 121(6), 1270-1280.

Publication 2014

Escherichia coli 0111:B4

Body weight Endotoxin shock Escherichia coli Intra peritoneal injection Lipopolysaccharide Mice Normal saline

Corresponding Organization : Harvard University

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Variable analysis

independent variables

Lipopolysaccharide (15 mg/kg body weight) (Escherichia coli 0111:B4, Sigma, St Louis, MO)

dependent variables

Not explicitly mentioned

control variables

Administration of 1 ml of pre-warmed normal saline

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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