Western blot assays were used according to standard techniques as previously reported (40 (link)). Antibodies against CD146 (ab75769; Abcam) and β-actin (#3700; CST) were used.
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Protocol detail
Western Blot Analysis of CD146
Corresponding Organization : Nankai University
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Protocol cited in 1 other protocol
Variable analysis
independent variables
- Manipulated variable not explicitly mentioned
- CD146 protein expression
- β-actin protein expression
- Controlled variables not explicitly mentioned
- Positive control: β-actin
- Negative control: Not specified
Annotations
This protocol is too short. Unable to provide accurate annotations
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