Episcleral Vein Cautery in Rats

Briefly, in deeply anesthetized rats (40 mg/kg thiopental and 10 mg/kg diazepam, given intraperitoneally), two drops in each eye of tropicamide (Mydriacyl 1% Alcon, Camberley, UK) (pupil dilatation) and tetracaine (Tetracaine, Pliva, Zagreb, Croatia) (local anesthesia) were administered. Two dorsal episcleral veins and one temporal episcleral vein were isolated from the surrounding tissues, as described [45 (link)]. A cautery was specifically applied to the selected vein with extra caution to spare surrounding tissue.

Free full text: Click here

Kralj T., Kokot A., Zlatar M., Masnec S., Kasnik Kovac K., Milkovic Perisa M., Batelja Vuletic L., Giljanovic A., Strbe S., Sikiric S., Balog S., Sontacchi B., Sontacchi D., Buljan M., Lovric E., Boban Blagaic A., Skrtic A., Seiwerth S, & Sikiric P. (2021). Stable Gastric Pentadecapeptide BPC 157 Therapy of Rat Glaucoma. Biomedicines, 10(1), 89.

Publication 2021

Mydriacyl

Cautery Diazepam Eye drops Local anesthesia Mydriacyl Pupil dilatation Rats Tetracaine Thiopental Tissue Vein

Corresponding Organization : University of Osijek

Other organizations : Klinički bolnički centar Osijek, Polytechnic of Međimurje in Čakovec

Top 5 similar protocols

Protocol cited in 6 other protocols

Variable analysis

independent variables

Administration of thiopental (40 mg/kg) and diazepam (10 mg/kg) intraperitoneally to induce deep anesthesia
Application of tropicamide (1% solution) and tetracaine eye drops to dilate the pupils and provide local anesthesia
Isolation of two dorsal episcleral veins and one temporal episcleral vein from the surrounding tissues
Cautery application to the selected vein with extra caution to spare surrounding tissue

dependent variables

Not explicitly mentioned

control variables

Anesthesia level (40 mg/kg thiopental and 10 mg/kg diazepam)
Pupil dilation (tropicamide 1% eye drops)
Local anesthesia (tetracaine eye drops)

controls

No positive or negative controls are explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!