Phytochemical Analysis Protocol

Acetonitrile, water (HPLC), methylene chloride, ethyl acetate, acetone, ethyl ether, albumin standard, 1-chloro-2,4-dinitrobenzene, nicotinamide adenine dinucleotide phosphate (NADPH) and paraffin wax was obtained from Merck (Germany). Streptozotocin (STZ) was obtained from Sigma Chemicals Co (St. Louis, MO, USA). Referent HPLC standards (chlorogenic acid, vanillic acid, p-coumaric acid, rutin, hyperoside, iso quercetin, ellag-ic acid, kaempferol-3-O-glucoside, morin, resveratrol, quercetin, luteolin, kaempferol, gallic acid, cyanidin chloride, cynanidin-3-O-glucoside, cyanidin-3-O-rutinoside, pro-cyanidin B1, protocatechuic acid, protocatechuic acid ethyl ester, p-hydroxybenzoic acid, epicatechin and phloridzin) (HPLC grade, ≥99% purity) were obtained from Extrasynthese (Lyon, France). The other chemicals were purchased from Sigma-Aldrich, Schnelldorf, Germany.

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Vukmirović S., Ilić V., Tadić V., Čapo I., Pavlović N., Tomas A., Paut Kusturica M., Tomić N., Maksimović S, & Stilinović N. (2023). Comprehensive Analysis of Antioxidant and Hepatoprotective Properties of Morus nigra L.. Antioxidants, 12(2), 382.

Publication 2023

Acetonitrile methylene chloride 1-chloro-2,4-dinitrobenzene paraffin wax Streptozotocin (STZ) chlorogenic acid vanillic acid p-coumaric acid rutin hyperoside isoquercetin ellag-ic acid kaempferol-3-O-glucoside resveratrol quercetin luteolin kaempferol gallic acid cyanidin chloride cyanidin-3-O-rutinoside pro-cyanidin B1 protocatechuic acid p-hydroxybenzoic acid epicatechin phloridzin

Acetone Acetonitrile Acid Albumin Chlorogenic acid Cyanidin Cyanidin 3 rutinoside Cyanidin chloride Dinitrobenzene Epicatechin Ester Ethyl acetate Ethyl ether Gallic acid Glucoside Hplc Hyperoside Isoquercetin Kaempferol Kaempferol 3 o glucoside Luteolin Methylene chloride Morin Nicotinamide adenine dinucleotide phosphate P coumaric acid P hydroxybenzoic acid Paraffin wax Phloridzin Protocatechuic acid Quercetin Resveratrol Rutin Streptozotocin Vanillic acid

Corresponding Organization : University of Novi Sad

Other organizations : Institute for Plant Protection and Environment, Klinički centar Vojvodine, Institute of Public Health of Vojvodina, University of Belgrade

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Variable analysis

independent variables

Acetonitrile
Methylene chloride
Ethyl acetate
Acetone
Ethyl ether
1-chloro-2,4-dinitrobenzene
Nicotinamide adenine dinucleotide phosphate (NADPH)
Paraffin wax
Streptozotocin (STZ)

dependent variables

Not explicitly mentioned

control variables

Water (HPLC)
Albumin standard
Referent HPLC standards (chlorogenic acid, vanillic acid, p-coumaric acid, rutin, hyperoside, isoquercetin, ellag-ic acid, kaempferol-3-O-glucoside, morin, resveratrol, quercetin, luteolin, kaempferol, gallic acid, cyanidin chloride, cynanidin-3-O-glucoside, cyanidin-3-O-rutinoside, pro-cyanidin B1, protocatechuic acid, protocatechuic acid ethyl ester, p-hydroxybenzoic acid, epicatechin and phloridzin)

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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