As described previously,32 (link) H37Rv-GFP was grown to an OD600 of
∼0.3 in 7H9 OADC and exposed to the drug at 1× or 10×
MIC. Every 24 h, over a period of 8 days, 200 μL of culture
was harvested, pelleted by centrifugation, and the supernatant was
transferred to a black, clear-bottom 96-well microtiter plate (Greiner
CellStar) and the fluorescence (excitation, 540 nm; emission, 590
nm) was measured using a SpectraMax i3x plate reader (Molecular Devices).
Fluorescence intensity was normalized by OD650 and standardized
to the value of the drug-free control for each sample.
∼0.3 in 7H9 OADC and exposed to the drug at 1× or 10×
MIC. Every 24 h, over a period of 8 days, 200 μL of culture
was harvested, pelleted by centrifugation, and the supernatant was
transferred to a black, clear-bottom 96-well microtiter plate (Greiner
CellStar) and the fluorescence (excitation, 540 nm; emission, 590
nm) was measured using a SpectraMax i3x plate reader (Molecular Devices).
Fluorescence intensity was normalized by OD650 and standardized
to the value of the drug-free control for each sample.
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