GRASP Genotypes for Synaptic Visualization
Corresponding Organization :
Other organizations : University of Bonn
Variable analysis
- Hug1.2lexA;lexAop-CD4::spGFP11
- GR66a-Gal4;UAS-CD4::spGFP1-10
- GFP reconstitution across synaptic partners (GRASP) method
- Larval CNS dissection
- Anti-mouse-GFP (Abcam, 1:500) primary antibody staining
- Anti-mouse-Alexa Fluor 488 (Invitrogen, 1:500) secondary antibody staining
- Imaging using ZEISS LSM 780 Laser scanning microscope with LCI Plan-Neofluar 25 × per 0.8 Imm Korr DIC M27
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
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