Genotypes used for GFP reconstitution across synaptic partners (GRASP) method19 (link) were: Hug1.2lexA;lexAop-CD4::spGFP11 and GR66a-Gal4;UAS-CD4::spGFP1-10. Larval CNS was dissected and stained with anti-mouse-GFP (Abcam, 1:500, secondary antibody was anti-mouse-Al488 (Invitrogen, 1:500)). Images were acquired using a ZEISS LSM 780 Laser scanning microscope with LCI Plan-Neofluar 25 × per 0.8 Imm Korr DIC M27.
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