Larval discs were dissected, fixed and stained followed by protocol as previous described [44 (link)]. Primary antibodies were: mouse-anti Cut (1:200), mouse anti-Repo (1:100), rat-anti-Elav (1:200), rat-anti E-Cadherin (1:25) from Developmental Studies Hybridoma Bank (DSHB, University of Iowa). The anti-cleaved caspase 3 (CC3) antibody [45 (link)] is from Cell Signaling Technology 9661S. Fluorescence conjugated secondary antibodies, including anti-HRP (1:300), were obtained from Jackson ImmunoResearch. Imaging procedures were acquired by LSM 510 Meta or LSM 780 confocal microscope (Zeiss).
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