A capture IgM ELISA assay (Venture Technologies, Sarawak, Malaysia) that utilizes inactivated antigens from JEV and DENV1–DENV4 was used to detect and distinguish between JEV- and DENV-specific IgM antibodies in CSF, and was performed as previously described [23] (link).
A chemiluminescent microparticle immunoassay (CMIA, Abbott diagnostic division, Lake Forest, Illinois 60045, USA) was used for the detection of IgM antibodies to rubella virus in serum, and was performed according to the manufacturer's instructions.
For detection of EBV antibodies in serum, an IgG/IgM ELISA (Enzygnost, Dade-Behring, Marburg, Germany) using recombinant viral capsid antigen (VCA) and EBV nuclear antigen (EBNA) was used, and was performed according to the manufacturer's instructions.
Detection of CMV-specific IgM and IgG antibodies in sera of patients that had CMV DNA detected in CSF samples were done with use of Abbott Architect CMV IgM/IgG assays (Abbott diagnostic division), and was performed according to the manufacturer's instructions.
A chemiluminescent microparticle immunoassay (CMIA, Abbott diagnostic division, Lake Forest, Illinois 60045, USA) was used for the detection of IgM antibodies to rubella virus in serum, and was performed according to the manufacturer's instructions.
For detection of EBV antibodies in serum, an IgG/IgM ELISA (Enzygnost, Dade-Behring, Marburg, Germany) using recombinant viral capsid antigen (VCA) and EBV nuclear antigen (EBNA) was used, and was performed according to the manufacturer's instructions.
Detection of CMV-specific IgM and IgG antibodies in sera of patients that had CMV DNA detected in CSF samples were done with use of Abbott Architect CMV IgM/IgG assays (Abbott diagnostic division), and was performed according to the manufacturer's instructions.
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