GC-MS Analysis of Volatile Oil Compounds

A GC-MS 7890A equipped with an Agilent 5975C quadrupole mass spectrometer controlled by Enhanced ChemStation MSD ChemStation E.01.00.237 (Agilent, Santa Clara, CA) was used to analyze compounds in the extracted volatile oil. Gas chromatography conditions: column was a HP INNOWax (30 m×0.25 mm×0.25 µm), inlet temperature 200°C, column flow 1.0 mL/min, ion source temperature 200°C, injection volume 1 µL and split ratio 10:1. Temperature-programmed conditions: initial temperature 80°C, with 10°C/min rise to 150°C, maintained 1 min, then with 5°C/min rise to 200°C, maintained 3 min, then with 5°C/min rise to 250°C and the carrier gas was He [42] (link)–[44] (link). The content was calculated by peak area normalization method.

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Yin Y., He Y., Liu W., Gan L., Fu C., Jia H, & Li M. (2014). The Durative Use of Suspension Cells and Callus for Volatile Oil by Comparative with Seeds and Fruits in Capparis spinosa L. PLoS ONE, 9(11), e113668.

Publication 2014

GC-MS 7890A Agilent 5975C quadrupole mass spectrometer

Gas chromatography Gc ms Volatile oil

Corresponding Organization : Hubei University

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Variable analysis

independent variables

Temperature-programmed conditions: initial temperature 80°C, with 10°C/min rise to 150°C, maintained 1 min, then with 5°C/min rise to 200°C, maintained 3 min, then with 5°C/min rise to 250°C

dependent variables

Content of compounds in the extracted volatile oil

control variables

Column: HP INNOWax (30 m×0.25 mm×0.25 µm)
Inlet temperature: 200°C
Column flow: 1.0 mL/min
Ion source temperature: 200°C
Injection volume: 1 µL
Split ratio: 10:1
Carrier gas: He

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