Fatty Acid Profiling in Mouse Tissues

Fatty acid profiles of mouse diets, tail and intestinal tissues were analyzed by gas chromatography as described previously60 (link). Briefly, tissue or food samples were grounded to powder under liquid nitrogen and subjected to total lipid extraction and fatty acid methylation by 14% boron trifluoride (BF3)-methanol reagent (Sigma-Aldrich) at 100 °C for 1 h. Fatty acid methyl esters were analyzed using a fully automated HP5890 gas chromatography system equipped with a flame-ionization detector (Agilent Technologies, Palo Alto, CA). The fatty acid peaks were identified by comparing their relative retention times with the commercial mixed standards (NuChek Prep, Elysian, MN), and area percentage for all resolved peaks was analyzed by using a PerkinElmer M1 integrator.

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Kaliannan K., Wang B., Li X.Y., Kim K.J, & Kang J.X. (2015). A host-microbiome interaction mediates the opposing effects of omega-6 and omega-3 fatty acids on metabolic endotoxemia. Scientific Reports, 5, 11276.

Publication 2015

BF3)-methanol reagent HP5890 M1 integrator

Boron trifluoride Diets Esters Fatty acid Flame ionization Food Gas chromatography Intestinal Lipid Methanol Methylation Mouse Nitrogen Powder Retention Tail Tissue

Corresponding Organization :

Other organizations : Massachusetts General Hospital, Harvard University

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Protocol cited in 2 other protocols

Variable analysis

independent variables

Mouse diets

dependent variables

Fatty acid profiles of mouse tail and intestinal tissues
Area percentage for all resolved peaks

control variables

Tissue or food samples were grounded to powder under liquid nitrogen
Fatty acid methylation by 14% boron trifluoride (BF3)-methanol reagent (Sigma-Aldrich) at 100 °C for 1 h
Fatty acid methyl esters were analyzed using a fully automated HP5890 gas chromatography system equipped with a flame-ionization detector (Agilent Technologies, Palo Alto, CA)
The fatty acid peaks were identified by comparing their relative retention times with the commercial mixed standards (NuChek Prep, Elysian, MN)

controls

Commercial mixed standards (NuChek Prep, Elysian, MN) were used as positive controls to identify the fatty acid peaks

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