Tissue-Specific Gene Expression Analysis

Total RNA was extracted using the RNAprep pure Plant Kit (TIANGEN, DP432) and reverse transcription using the EvoM-MLV RT Kit with gDNA Clean for qPCR (Accurate Biology, AG11705). The quantitative real-time PCR (qPCR) was performed using the 2× All-in-one ™-qPCR Mix (Genecopoeia, Qp001-01). The specific primers were designed using Primer 3 Input software (version 4.1.0). The internal reference gene was TIP41 (Fan et al., 2013 (link)); primers are shown in the Supplementary Table S4. Expression patterns were analyzed by transcriptomic data and real-time quantitative PCR to synthetically screen candidate tissue-specific genes for in situ hybridization analysis (Wang et al., 2017 (link); Li et al., 2018a (link)). Gene expression heat mapping was performed using TBtools software (Chen et al., 2020 (link)).