Culturing Glioblastoma Stem-like Cells

Stem-like RN1, JK2, RKI1 and HW1 cells were derived from glioblastoma specimens [39 (link),40 (link)]. Cells were cultured in KnockOut DMEM/F-12 basal medium supplemented with StemPro NSC SFM supplement, 2 mM GlutaMAX-ICTS, 20 ng/mL EGF, 10 ng/mL FGF-β and Antibiotic–Antimycotic solution (all Life Technologies) as adherent cells on flasks coated with MatriGel Matrix (Corning Life Sciences, Cat# 354234, Corning, NY, USA). The protocols were approved by the Human Ethics Committee of the Royal Brisbane and Women’s Hospital (RBWH 2004/161). All cell cultures were routinely tested for mycoplasma contamination and the cumulative length of culturing did not exceed 15 passages.

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Phoa A.F., Recasens A., Gurgis F.M., Betts T.A., Menezes S.V., Chau D., Nordfors K., Haapasalo J., Haapasalo H., Johns T.G., Stringer B.W., Day B.W., Buckland M.E., Lalaoui N, & Munoz L. (2020). MK2 Inhibition Induces p53-Dependent Senescence in Glioblastoma Cells. Cancers, 12(3), 654.

Publication 2020

StemPro NSC SFM supplement GlutaMAX-ICTS EGF FGF-β Antibiotic–Antimycotic solution MatriGel Matrix

Antibiotic Cell cultures Cells Cultured medium Ethics committee Glioblastoma Human Icts Matrigel Mycoplasma Stem Supplement Women

Corresponding Organization : University of Sydney

Other organizations : Walter and Eliza Hall Institute of Medical Research, University of Melbourne, Tampere University, Tampere University Hospital, SickKids Foundation, Hospital for Sick Children, Fimlab (Finland), The Kids Research Institute Australia, Princess Margaret Hospital for Children, QIMR Berghofer Medical Research Institute

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Variable analysis

independent variables

Cell type (Stem-like RN1, JK2, RKI1 and HW1 cells)

dependent variables

Not explicitly mentioned

control variables

Culture medium (KnockOut DMEM/F-12 basal medium supplemented with StemPro NSC SFM supplement, 2 mM GlutaMAX-ICTS, 20 ng/mL EGF, 10 ng/mL FGF-β and Antibiotic–Antimycotic solution)
Cell culture substrate (MatriGel Matrix coated flasks)
Mycoplasma contamination (routinely tested)
Passage number (cumulative length of culturing did not exceed 15 passages)

positive controls

Not mentioned

negative controls

Not mentioned

Annotations

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