Non-targeted Metabolomics Analysis of Cell-free Supernatant

To compare the metabolites of the cell-free supernatant, a non-targeted metabonomic was performed. The sample preparation referred to our previous study [13 (link)]. LC-MS/MS determination was performed by Thermo UHPLC-Q Exactive HF-X Mass Spectrometer. After separation by an HSS T3 column, the samples were subjected to MS/MS detection for compound identification. Q Exactive HF-X mass spectrometer was operated as follows: −3500 V in negative mode and 3500 V in positive mode, capillary temperature at 325 °C, heater temperature at 425 °C, MS resolution at 60,000, MS/MS resolution at 75,000. Mass range was set to 70–1050. After determination, Metlin (https://metlin.scripps.edu/, accessed on 23 May 2023) and HMDB (http://www.hmdb.ca/, accessed on 3 June 2023) were used to identify the metabolites. The data were analyzed by Majorbio cloud platform (https://cloud.majorbio.com, accessed on 4 June 2023). Detailed information about the data filtered, data normalized, and data analyzed can be found in our previous study [13 (link)].

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Sun Z., Zhao Z., Fang B., Hung W., Gao H., Zhao W., Lan H., Liu M., Zhao L, & Zhang M. (2023). Effect of Thermal Inactivation on Antioxidant, Anti-Inflammatory Activities and Chemical Profile of Postbiotics. Foods, 12(19), 3579.

Publication 2023

UHPLC-Q Exactive HF-X Mass Spectrometer cloud platform

Capillary Ms ms

Corresponding Organization : Beijing Technology and Business University

Other organizations : China Agricultural University, Hefei University of Technology

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Metabolites of the cell-free supernatant

control variables

Sample preparation procedure was referred to previous study [13]
LC-MS/MS determination parameters: Thermo UHPLC-Q Exactive HF-X Mass Spectrometer, HSS T3 column, −3500 V in negative mode and 3500 V in positive mode, capillary temperature at 325 °C, heater temperature at 425 °C, MS resolution at 60,000, MS/MS resolution at 75,000, mass range set to 70–1050

controls

Not mentioned
Not mentioned

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