Immunofluorescence Analysis of Primary Hepatocytes

Primary hepatocytes were fixed in 4% paraformaldehyde and made into smear. Immunofluorescence was performed as previous described [9 (link)]. Albumin antibody (1:100, Santa Cruz Biotechnology, Santa Cruz, CA, USA), CD31 antibody (1:50, Santa Cruz Biotechnology, Santa Cruz, CA, USA), Laminin antibody (1:100, Abcam, Cambridge, UK), and Nestin antibody (1:50, Chemicon, Billerica, MA, USA) were used. FITC-conjugated donkey anti-goat antibody or Cy3-conjugated goat anti-rabbit antibody was used as secondary antibodies (1:100, Jackson Immuno-Research, West Grove, PA). At last, nuclei were stained with DAPI.

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Chang N., Tian L., Ji X., Zhou X., Hou L., Zhao X., Yang Y., Yang L, & Li L. (2019). Single-Cell Transcriptomes Reveal Characteristic Features of Mouse Hepatocytes with Liver Cholestatic Injury. Cells, 8(9), 1069.

Publication 2019

CD31 antibody Laminin antibody Nestin antibody FITC-conjugated donkey anti-goat antibody Cy3-conjugated goat anti-rabbit antibody

Albumin Anti antibody Antibodies Antibody Dapi Donkey Fitc Goat Hepatocytes Immunofluorescence Laminin Nestin Nuclei Paraformaldehyde Rabbit

Corresponding Organization : Capital Medical University

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Variable analysis

independent variables

Primary hepatocytes were fixed in 4% paraformaldehyde and made into smear

dependent variables

Immunofluorescence expression of albumin, CD31, laminin, and nestin

control variables

Primary hepatocytes
Fixation in 4% paraformaldehyde
Immunofluorescence protocol as previously described

controls

Positive control: not specified
Negative control: not specified

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