Genetic Diversity of Forsythia suspensa

The specimen used for de novo assembly was collected from the Zhengzhou Botanical Garden (voucher no. 2018LIFS021). The 300 F. suspensa samples were collected from 15 geographically isolated locations (voucher No. 2018LIFS001–2018LIFS015) covering its current distribution range in China (Fig. 1a; Table 3). In addition, two samples of Forsythiaviridissima and Jasminum nudiflorum were collected from Henan Agricultural University. The population samples used for SLAF sequencing were placed in silica gel at room temperature after collection. All samples included in this study were used according to Chinese regulations. All voucher specimens were identified by Dr. Yong Li.Table 3

Details of the population locations of Forsythia suspensa sampled in China

Population no. and code	Locations	Lat.(N)/Long.(E)	N
Northwest group
1. SXWL	Wulaofeng, Shanxi	34.81/110.58	20
2. SXHM	Hua Mt., Shaanxi	34.52/110.08	20
Southwest group
3. SXLJ	Laojun Mt., Shaanxi	34.33/110.20	20
4. HBWD	Wudang Mt., Hubei	32.42/110.01	20
5. HNLY	Longyuwan, Henan	33.67/111.79	20
6. HNLJ	Laojieling, Henan	33.66/111.77	20
Eastern group
7. SDBD	Baodugu, Shandong	34.99/117.71	20
8. SDTM	Tai Mt., Shandong	36.21/117.12	20
9. SDMM	Meng Mt., Shandong	35.56/117.97	20
Northern group
10. SXLK	Lingkong Mt., Shanxi	36.59/112.08	20
11. HBWZ	Wuzhi Mt., Hebei	36.51/113.65	20
12. HNJL	Jiulian Mt., Henan	35.64/113.55	20
13. HNSM	Song Mt., Henan	34.50/113.02	20
Southern group
14. HNTB	Tongbai Mt., Henan	32.36/113.36	20
15. HNJG	Jigong Mt., Henan	31.80/114.08	20

N number of individuals