Western Blot Analysis of Cellular Proteins

Western blot analysis was performed as described previously [30 (link), 31 (link)]. The following antibodies were used: rat anti-NAMPT clone 14A.5 (1:1,000, Millipore), rabbit anti-GAPDH (1:20,000, Trevigen), mouse anti-NAPRT(B-8) (1:1,000, Santa Cruz Biotechnology), rabbit anti-POTEE antibody (1:200, Abcam), mouse monoclonal anti-beta actin clone AC-15 (1:20,000, Sigma-aldrich), horseradish peroxidase-linked anti-rat IgG (1:20,000, GE Healthcare), horseradish peroxidase-linked anti-rabbit IgG (1:20,000, GE Healthcare), and horseradish peroxidase-linked whole antibody anti-mouse IgG (1:20,000, GE Healthcare).

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Ogino Y., Sato A., Uchiumi F, & Tanuma S.I. (2018). Cross resistance to diverse anticancer nicotinamide phosphoribosyltransferase inhibitors induced by FK866 treatment. Oncotarget, 9(23), 16451-16461.

Publication 2018

rabbit anti-GAPDH horseradish peroxidase-linked anti-rabbit IgG horseradish peroxidase-linked whole antibody anti-mouse IgG

Anti antibody Anti igg Antibodies Antibody Beta actin Clone Gapdh Horseradish peroxidase Mouse Nampt Rabbit Western blot analysis

Corresponding Organization :

Other organizations : Tokyo University of Science

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