Western Blot Analysis of Protein Levels

N2A cells and brain tissues were homogenized in 10 volumes of radioimmunoprecipitation assay (RIPA) buffer (Thermo Scientific, Waltham, MA, USA) containing protease inhibitors (Sigma-Aldrich, St. Louis, MO, USA) and phosphatase inhibitors (Sigma-Aldrich, St. Louis, MO, USA). A Western blot analysis was performed as previously described [64 (link)]. The membranes were immunoblotted with primary antibodies, followed by incubation with horseradish peroxidase-conjugated antirabbit and antimouse secondary antibodies (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA, USA). The antibodies used in this study are listed in Supplementary Table S2. The band densities were measured using ImageJ software (version 1.53, National Institutes of Health, Bethesda, MD, USA) and normalized to the density of actin.