Fluorescent Protein Tagged PILS and CHER Localization

The following published lines were used:p35S::PILS5-GFP (6 (link)), p35S::GFP-PILS3 (1 ), p35S::PILS6-GFP (2 (link)), pPILS3::PILS3-GFP (1 ), pDR5::GFP (29 (link)), pils2 pils5 (6 (link)), pils2 pils3 pils5 (7 (link)), cher1-4 (30 (link)), pCHER1::CHER1-YFP (9 (link)), p35S::GFP-HDEL (31 (link)), p35S::DER1-mScarlet (8 (link)) (all in Col-0 background). Seeds were stratified at 4 °C for 2 d in the dark. Seedlings were grown vertically on half Murashige and Skoog medium (1/2 MS salts (Duchefa), pH 5.9, 1% sucrose, and 0.8% agar). Plants were grown under long-day (16 h light/8 h dark) or under dark conditions at 20 to 22 °C.

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Waidmann S., Béziat C., Ferreira Da Silva Santos J., Feraru E., Feraru M.I., Sun L., Noura S., Boutté Y, & Kleine-Vehn J. (2023). Endoplasmic reticulum stress controls PIN-LIKES abundance and thereby growth adaptation. Proceedings of the National Academy of Sciences of the United States of America, 120(31), e2218865120.

Publication 2023

MS salts

Corresponding Organization :

Other organizations : University of Freiburg, BOKU University, Laboratoire de Biogenèse Membranaire

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Variable analysis

independent variables

P35S::PILS5-GFP
P35S::GFP-PILS3
P35S::PILS6-GFP
PPILS3::PILS3-GFP
PDR5::GFP
Pils2 pils5
Pils2 pils3 pils5
Cher1-4
PCHER1::CHER1-YFP
P35S::GFP-HDEL
P35S::DER1-mScarlet

dependent variables

Not explicitly mentioned

control variables

Background: Col-0
Stratification: 4 °C for 2 d in the dark
Growth conditions: Vertically on half Murashige and Skoog medium (1/2 MS salts (Duchefa), pH 5.9, 1% sucrose, and 0.8% agar), under long-day (16 h light/8 h dark) or under dark conditions at 20 to 22 °C

positive controls

None specified

negative controls

None specified

Annotations

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