Salmonella Genomic DNA Extraction and Sequencing

Genomic DNA of all 105 selected Salmonella isolates was extracted from cultures incubated in Luria-Bertani (LB) broth overnight using a FastPure bacterial DNA isolation minikit (Vazyme Biotech Co., Ltd., Nanjing, China) according to the manufacturer’s instructions and quantified using a Nanodrop 1000 instrument (Thermo Fisher Scientific, USA). Genomic DNA of eligible quality was sequenced on the Illumina NovaSeq 6000 platform by Novogene (Beijing, China), as described previously (6 (link)).

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Li Y., Kang X., Ed-Dra A., Zhou X., Jia C., Müller A., Liu Y., Kehrenberg C, & Yue M. (2022). Genome-Based Assessment of Antimicrobial Resistance and Virulence Potential of Isolates of Non-Pullorum/Gallinarum Salmonella Serovars Recovered from Dead Poultry in China. Microbiology Spectrum, 10(4), e00965-22.

Publication 2022

FastPure bacterial DNA isolation minikit Nanodrop 1000 instrument NovaSeq 6000 platform

Bacterial dna Genomic Isolation Salmonella

Corresponding Organization : National Clinical Research Center for Digestive Diseases

Other organizations : University of Giessen, Shandong Academy of Agricultural Sciences

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Variable analysis

independent variables

Genomic DNA of all 105 selected Salmonella isolates

dependent variables

Genomic DNA sequencing of Salmonella isolates

control variables

Incubation of Salmonella isolates in Luria-Bertani (LB) broth overnight
DNA extraction using a FastPure bacterial DNA isolation minikit
DNA quantification using a Nanodrop 1000 instrument
Genomic DNA sequencing on the Illumina NovaSeq 6000 platform

controls

No positive or negative controls were explicitly mentioned in the provided information.

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