The protocols followed in this study were developed by Malavolta et al.18 Different techniques were used for different minerals:

Atomic absorption spectrophotometry for Sodium (Na), Zinc (Zn), Manganese (Mn), Iron (Fe), Calcium (Ca), Magnesium (Mg), and Potassium (K).

Colorimetry of aluminum: Aluminum (Al)

Silver nitrate titrimetry: Chlorine (Cl)

Metavanadate colorimetry: Phosphorus (P)

Barium sulfate turbidimetry: Sulfur (S)

Semi-micro-Kjeldahl: Nitrogen (N)

Free full text: Click here