Cell Viability Assay via MTT

The in vitro cell viability test was performed with a MTT assay as described previously [43 (link), 44 (link)]. 24 h post transfection, cells were seeded in 96-well culture plates (4 × 10³/well) for 12 h, 24 h, 36 h, 48 h, and 60 h (in triplicate for each condition). 20 µl MTT (Sigma, Saint Louis, USA) stock solution (5 mg/ml) was added to each well and incubated for 2–3 h. Then, the supernatant was removed, followed by the solubilisation of MTT crystals using DMSO and the absorbance was measured at 570 nm using plate reader BioTek Eon (BioTek, Winooski, USA).

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Ashok C., Ahuja N., Natua S., Mishra J., Samaiya A, & Shukla S. (2021). E2F1 and epigenetic modifiers orchestrate breast cancer progression by regulating oxygen-dependent ESRP1 expression. Oncogenesis, 10(8), 58.

Publication 2021

MTT

Assay Cell viability Cells Dmso In vitro test Transfection

Corresponding Organization :

Other organizations : Indian Institute of Science Education and Research, Bhopal, Bansal Institute Of Research Technology & Science

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Variable analysis

independent variables

Time points: 12 h, 24 h, 36 h, 48 h, and 60 h post transfection

dependent variables

Cell viability as measured by MTT assay

control variables

Cell seeding density: 4 × 10^3 cells/well
MTT assay procedure: 20 µl MTT stock solution (5 mg/ml) added to each well and incubated for 2-3 h, followed by solubilisation of MTT crystals using DMSO and absorbance measurement at 570 nm

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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