Amino Acid Sequencing and BLAST Analysis

Amino acid sequencing was performed as previously described [13 (link)] on the Applied Biosystems (Foster City, CA) Procise®494 protein sequencer using standard Edman sequencing. BLAST (basic local alignment sequence tool) software was used to search for amino acid homology between observed amino acid sequence data and known human protein sequences.

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Zimering M.B., Mirkovic N., Pandya M., Zimering J., Behnke J., Thakker-Varia S., Alder J, & Donnelly R. (2016). Toxic Immunoglobulin Light Chain Autoantibodies are Associated with a Cluster of Severe Complications in Older Adult Type 2 Diabetes. Journal of endocrinology and diabetes, 3(1), 10.15226/2374-6890/3/1/00141.

Publication 2016

Procise®494

Amino acid Basic sequence Human Human protein Protein Protein sequences

Corresponding Organization : Veterans Biomedical Research Institute

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Variable analysis

independent variables

Amino acid sequencing protocol

dependent variables

Observed amino acid sequence data

control variables

Standard Edman sequencing

controls

No positive or negative controls are explicitly mentioned.

Annotations

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