Cytokine Profiling of Mouse Microglia

The Proteome Profiler Mouse Cytokine Array Kit (Panel A; R&D Systems, USA) was used to analyze the cytokine arrays using filtered supernatant collected from primary mouse microglial cultures at 48 h incubation in the presence or absence of CSF-1RAb LPS and ATP. Assays were carried out according to the manufacturer’s protocol as previously described (37 (link)). The array membranes were imaged with film developer (SRX-101A, Konica Minolta, USA) and the results were analyzed using ImageJ software.

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Tang Y., Xiao Z., Pan L., Zhuang D., Cho K.S., Robert K., Chen X., Shu L., Tang G., Wu J., Sun X, & Chen D.F. (2020). Therapeutic Targeting of Retinal Immune Microenvironment With CSF-1 Receptor Antibody Promotes Visual Function Recovery After Ischemic Optic Neuropathy. Frontiers in Immunology, 11, 585918.

Publication 2020

SRX-101A

Assays Cytokine Membranes Microglial Mouse Proteome

Corresponding Organization : Harvard University

Other organizations : Hong Kong Polytechnic University, First Hospital of Shijiazhuang

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Variable analysis

independent variables

Presence or absence of CSF-1RAb LPS and ATP

dependent variables

Cytokine levels in the filtered supernatant collected from primary mouse microglial cultures

control variables

Primary mouse microglial cultures
Incubation time (48 h)

controls

Positive control: Not specified
Negative control: Not specified

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