Cellular RNA Extraction and Stress PCR Profiling

Preparation of total cellular RNA, reverse transcription, and Human Oxidative Stress RT²Profiler™ PCR Expression Array (SuperArray) profiling were performed as described in ‘Supplementary Materials and Methods’ online (Lamore et al., 2010b (link); Qiao et al., 2013 (link)).

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Park S.L., Justiniano R., Williams J.D., Cabello C.M., Qiao S, & Wondrak G.T. (2014). The tryptophan-derived endogenous arylhydrocarbon receptor ligand 6-formylindolo[3,2-b]carbazole (FICZ) is a nanomolar UVA-photosensitizer in epidermal keratinocytes. The Journal of investigative dermatology, 135(6), 1649-1658.

Publication 2014

Cellular Human Oxidative stress Reverse transcription

Corresponding Organization : University of Arizona

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Protocol cited in 7 other protocols

Variable analysis

independent variables

Preparation of total cellular RNA
Reverse transcription
Human Oxidative Stress RT²Profiler™ PCR Expression Array (SuperArray) profiling

dependent variables

Measured outcomes of the Human Oxidative Stress RT²Profiler™ PCR Expression Array (SuperArray) profiling

control variables

Not explicitly mentioned

controls

Positive and negative controls specified by the authors in the Supplementary Materials and Methods section

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