PET Imaging of Radiotracer Uptake in Rats

The general procedure for PET studies was described previously^{39 (link), 41 (link)} with minor modification in this work. Briefly, PET scans were carried out by an Inveon PET scanner (Siemens Medical Solutions, Knoxville, TN, USA). Sprague-Dawley rats were kept under anesthesia using 1–2% (v/v) isoflurane during the scan. The radiotracer (ca. 1 mCi / 150 μL) was injected into the tail vein via a preinstalled catheter. A dynamic scan in 3D list mode was acquired for 90 min. For pretreatment studies, a solution of KML29 (3 mg/kg) in 300 μL saline containing 10% ethanol and 5% Tween^® 80 was injected via the pre-embedded tail vein catheter at 30 min prior to tracer injection. As we previously reported,^{39 (link), 77 (link), 78 (link)} the PET dynamic images were reconstructed using ASIPro VW software (Analysis Tools and System Setup/Diagnostics Tool, Siemens Medical Solutions). Volumes of interest, including the whole brain, hippocampus, cerebral cortex, cerebellum, striatum, thalamus, and pons were placed using ASIPro software. The radioactivity was decay-corrected and expressed as the standardized uptake value. SUV = (radioactivity per mL tissue / injected radioactivity) × body weight.

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Chen Z., Mori W., Deng X., Cheng R., Ogasawara D., Zhang G., Schafroth M.A., Dahl K., Fu H., Hatori A., Shao T., Zhang Y., Yamasaki T., Zhang X., Rong J., Yu Q., Hu K., Fujinaga M., Xie L., Kumata K., Gou Y., Chen J., Gu S., Bao L., Wang L., Lee Collier T., Vasdev N., Shao Y., Ma J.A., Cravatt B.F., Fowler C., Josephson L., Zhang M.R, & Liang S.H. (2019). Design, synthesis and evaluation of reversible and irreversible monoacylglycerol lipase positron emission tomography (PET) tracers using a ‘tail switching’ strategy on a piperazinyl azetidine skeleton. Journal of medicinal chemistry, 62(7), 3336-3353.

Publication 2019

Inveon PET scanner ASIPro VW software

Anesthesia Body weight Brain Catheter Cerebellum Cerebral cortex Diagnostics Ethanol Hippocampus Isoflurane Pet scans Pons Radioactivity Saline Scan Sprague dawley rats Striatum Tail Thalamus Tissue Tween 80 Vein

Corresponding Organization : National Institutes for Quantum and Radiological Science and Technology

Other organizations : Tianjin University, Scripps Research Institute, University of Oklahoma, Umeå University

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Variable analysis

independent variables

Pretreatment with KML29 (3 mg/kg) in 300 μL saline containing 10% ethanol and 5% Tween® 80 injected via the pre-embedded tail vein catheter at 30 min prior to tracer injection

dependent variables

Standardized uptake value (SUV) in the whole brain, hippocampus, cerebral cortex, cerebellum, striatum, thalamus, and pons

control variables

Sprague-Dawley rats kept under anesthesia using 1–2% (v/v) isoflurane during the scan
Radiotracer (ca. 1 mCi / 150 μL) injected into the tail vein via a preinstalled catheter
Dynamic PET scan in 3D list mode acquired for 90 min

controls

No positive or negative controls were explicitly mentioned in the provided information.

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