We processed mouse brains and cut 10-μm-thick coronal sections for immunohistochemistry as described previously (13 (link)), using phospho (p)-Akt (catalog #4060; Cell Signaling Technology), pStat3 (catalog #9131; Cell Signaling Technology), protein S6 (pS6) antibodies (catalog #4858; Cell Signaling Technology), and cFos antibody (catalog #2250; Cell Signaling Technology).
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Immunohistochemistry of Mouse Brain
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Corresponding Organization : Columbia University Irving Medical Center
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Variable analysis
independent variables
- Brain sections
- Phospho (p)-Akt expression
- PStat3 expression
- Protein S6 (pS6) expression
- CFos expression
- Coronal sections
- Section thickness (10-μm)
- Immunohistochemistry protocol
- No positive or negative controls were explicitly mentioned.
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