Of each fresh preparation of ffEVs, 3 µL were fixed with 3 µL of 2% glutaraldehyde solution in PBS at room temperature. TEM was performed with 3 µL of fixed EVs as described earlier [45 (link)]. Micrographs were obtained using a Hitachi HT 7700 Elexience and a JEM 1011 TEM (JEOL, Japan) electron microscopes with digital camera. Images were used for measuring EV sizes with Fiji software [93 (link)].
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