Housing and Genetic Mouse Models

Animals were housed at 22–24°C on a 12:12 light/dark cycle (light cycle: 6:00 a.m. to 6:00 p.m.) with standard mouse chow (Teklad F6 Rodent Diet 8664; Harlan Teklad) and water provided ad libitum, unless specified otherwise. Adult male and female mice (8–16 weeks old) were used for experiments. Mice were maintained on a mixed background. Transgenic mouse strains used: Avp-IRES-Cre (Pei et al., 2014 (link)), Slc17a6-IRES-Cre (Vglut2-IRES-Cre) (Vong et al., 2011 (link)) (Jackson Labs Stock 016963), Slc32a1-IRES-Cre (Vgat-IRES-Cre) (Vong et al., 2011 (link)) (Jackson Labs stock 016962), Nos1-IRES-Cre (Jackson Labs Stock 017526), Slc17a6-IRES-Flp (Vglut2-IRES2-FLPo-D) (Jackson Labs Stock 030212), Slc32a1-IRES-Flp (Vgat-IRES2-FLPo-D) (Jackson Labs Stock 031331), Th-Cre (Savitt et al., 2005 (link)) (Jackson Labs Stock 008601), Dbh-Flp (MMRRC 041575), Avp-GFP (MMRRC 015858-UCD), Agrp-IRES-Cre (Tong et al., 2008 (link)) (Jackson Labs Stock 012899), and Pomc-IRES-Cre (Fenselau et al., 2017 (link)).

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Kim A., Madara J.C., Wu C., Andermann M.L, & Lowell B.B. (2021). Neural basis for regulation of vasopressin secretion by anticipated disturbances in osmolality. eLife, 10, e66609.

Publication 2021

Teklad F6 Rodent Diet 8664 Avp-IRES-Cre Slc17a6-IRES-Cre Slc32a1-IRES-Cre Nos1-IRES-Cre Th-Cre Agrp-IRES-Cre

Adult Agrp Animals Diet Female Ires Male Mice Nos1 Rodent Strains Transgenic mouse

Corresponding Organization :

Other organizations : Harvard University, Beth Israel Deaconess Medical Center

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Variable analysis

independent variables

Transgenic mouse strains used

dependent variables

Outcomes measured in the experiments

control variables

Temperature range (22–24°C)
Light/dark cycle (12:12)
Light cycle (6:00 a.m. to 6:00 p.m.)
Standard mouse chow and water provided ad libitum
Adult male and female mice (8–16 weeks old)
Mixed background of mice

controls

No positive or negative controls were explicitly mentioned in the protocol.

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