An Illumina paired-end library was constructed by the NEBNext® Ultra™ II DNA Library Preparation Kit (New England Biolabs (Beijing) Ltd., China) and sequenced on the Illumina HiSeq 4000 PE150 sequencing platform. Approximately 17.5 Gb of raw data was generated, and the raw sequence reads were filtered for primer/adaptor sequences and low-quality reads with the NGS QC Tool Kit [22 (link)]. Sequencing reads were assembled using SPAdes 3.6.1 software [23 (link)] with the parameter Kmer=95, and 198,659 contigs were finally obtained.
MISA software [24 (link), 25 (link)] was used to identify unique reads containing microsatellite repeats. The search was performed for a minimum repeat number of 5, 4, 3, 3 and 3 for di-, tri-, tetra-, penta-, and hexa-nucleotides, respectively. Primers were designed on the basis of flanking sequences of SSR microsatellite loci by using Primer 3. The parameters of primer design were set as follows: the primer size was between 18 and 25 bp with an optimal size of 22 bp, the annealing temperature was between 55 and 65 °C with the optimal temperature of 60 °C, the PCR product size was between 80 and 300 bp, and default values were selected for other settings.
MISA software [24 (link), 25 (link)] was used to identify unique reads containing microsatellite repeats. The search was performed for a minimum repeat number of 5, 4, 3, 3 and 3 for di-, tri-, tetra-, penta-, and hexa-nucleotides, respectively. Primers were designed on the basis of flanking sequences of SSR microsatellite loci by using Primer 3. The parameters of primer design were set as follows: the primer size was between 18 and 25 bp with an optimal size of 22 bp, the annealing temperature was between 55 and 65 °C with the optimal temperature of 60 °C, the PCR product size was between 80 and 300 bp, and default values were selected for other settings.
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