SOS Induction Measurement by Flow Cytometry

SOS induction measurements by flow cytometry was performed as previously described [17 (link),18 (link),25 (link)]. Briefly, overnight cultures were diluted 100-fold in MH or MH supplemented with subMIC tobramycin (0.02 μg/mL), subMIC ciprofloxacin (0.005 μg/mL) and/or AI-2 (10 μM) and were incubated overnight at 37 °C. Fluorescence was then measured in 100,000 cells on the Miltenyi MACSquant device. The fluorescence values in each condition were normalized to the fluorescence values obtained in MH.

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Carvalho A., Krin E., Korlowski C., Mazel D, & Baharoglu Z. (2021). Interplay between Sublethal Aminoglycosides and Quorum Sensing: Consequences on Survival in V. cholerae. Cells, 10(11), 3227.

Publication 2021

MACSquant device

Cells Ciprofloxacin Device Flow cytometry Fluorescence Sos induction Tobramycin

Corresponding Organization : Centre National de la Recherche Scientifique

Other organizations : Sorbonne Université, Université Sorbonne Nouvelle

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Variable analysis

independent variables

Presence of subMIC tobramycin (0.02 μg/mL)
Presence of subMIC ciprofloxacin (0.005 μg/mL)
Presence of AI-2 (10 μM)

dependent variables

Fluorescence measured in 100,000 cells

control variables

Overnight cultures diluted 100-fold in MH
Incubation overnight at 37 °C

controls

Fluorescence values in each condition were normalized to the fluorescence values obtained in MH (negative control)

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