Yeast strain YD116 [40 (link)] was transformed using the Frozen-EZ Yeast Transformation IITM Kit (Zymo Research Corp., Irvine, CA, USA), in accordance with the manufacturer’s instructions.
Quantitative β-galactosidase assay, β-galactosidase filter assay, and yeast growth assay were conducted as previously described [23 (link)]. In brief, a quantitative β-galactosidase activity using ONPG was quantified with the formula 1000 × OD420/(OD600 × assay time in min × assay volume in mL). β-Galactosidase filter assay was conducted using 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside as a substrate for 6 h. Yeast transformants grown on SD media lacking Trp and Ura were incubated for 3–5 days at 30 °C for the growth assay.
Quantitative β-galactosidase assay, β-galactosidase filter assay, and yeast growth assay were conducted as previously described [23 (link)]. In brief, a quantitative β-galactosidase activity using ONPG was quantified with the formula 1000 × OD420/(OD600 × assay time in min × assay volume in mL). β-Galactosidase filter assay was conducted using 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside as a substrate for 6 h. Yeast transformants grown on SD media lacking Trp and Ura were incubated for 3–5 days at 30 °C for the growth assay.
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