Comet Assay for DNA Damage

The extent of DNA damage was evaluated based on the protocol of alkaline single-cell gel electrophoresis (comet assay) described by Tice et al. 2000^{36 (link)}. Slides were examined under fluorescent microscope (Leica, Germany). For each sample, the degree of DNA migration was determined by capturing images for 50 nucleoids at 400× magnification using leica microscope camera. Analysis of comet parameters was done by using TriTek Comet Score™ Version 1.5 software. Tail length (TL), %DNA in tail and tail moment (TM) are used to evaluate the extent of DNA damage.

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Ghebryal L.N., Noshy M.M., El-Ghor A.A, & Eissa S.M. (2023). Comparative analysis of Acomys cahirinus and Mus musculus responses to genotoxicity, oxidative stress, and inflammation. Scientific Reports, 13, 3989.

Publication 2023

fluorescent microscope

Alkaline single cell gel electrophoresis Comet Dna damage Microscope Tail

Corresponding Organization : Cairo University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Tail length (TL)
Percentage of DNA in tail (%DNA in tail)
Tail moment (TM)

control variables

Fluorescent microscope (Leica, Germany)
Magnification (400×)
Software used for analysis (TriTek Comet Score™ Version 1.5)

controls

No positive or negative controls were explicitly mentioned.

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