In utero electroporation was performed as described previously [59 (link)]. Briefly, timed pregnant female mice from E14.5 of gestation were deeply anesthetized, and the uterine horns were gently exposed by laparotomy. The lateral ventricles of an embryonic brain were injected with plasmid DNA (2 μg/μl) and 0.001% fast green (Sigma-Aldrich) using a Picospritzer II (Parker Hannifin). Electroporation was achieved by placing two sterile forceps-type electrodes on opposing sides of the uterine sac around the embryonic head and applying a series of short electrical pulses using a BTX ECM 830 electroporator (Harvard Apparatus) (five pulses with 100 ms length separated by 900 ms intervals were applied at 45 V).
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In utero Electroporation of Embryonic Mouse Brains
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Electrical
Electroporation
Embryonic
Fast green
Forceps
Gestation
Head
Laparotomy
Lateral ventricles of brain
Mice
Plasmid
Pregnant female
Pulses
Sterile
Uterine
Uterine horns
Corresponding Organization : Kent State University
Other organizations : Korea Institute of Toxicology, Korea Research Institute of Chemical Technology, Hamad bin Khalifa University
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Variable analysis
independent variables
- Plasmid DNA injection into the lateral ventricles of the embryonic brain
- Electrical pulses applied using a BTX ECM 830 electroporator
- Not explicitly mentioned
- Timed pregnant female mice from E14.5 of gestation
- Inclusion of 0.001% fast green in the plasmid DNA injection
- Positive control: Not specified
- Negative control: Not specified
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