Protocol detail

Intradermal Bacterial and Leishmania Injection

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Briefly, the low (1.5×10³ CFU/mL) and high (1.5×10⁶ CFU/mL) doses of each bacterium were suspended in 10 μL of PBS either with or without L. major (1.5×10⁶/ml). Leishmania major alone and PBS were set as controls. The suspensions were injected intradermally into the right ear pinna of mice following anesthetizing with xylazine (10 mg/kg) and ketamine (80 mg/kg) (Table 1).

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Amni F., Maleki-Ravasan N., Nateghi-Rostami M., Hadighi R., Karimian F., Meamar A.R., Badirzadeh A, & Parvizi P. (2023). Co-infection of Phlebotomus papatasi (Diptera: Psychodidae) gut bacteria with Leishmania major exacerbates the pathological responses of BALB/c mice. Frontiers in Cellular and Infection Microbiology, 13, 1115542.

Publication 2023

Bacterium Ketamine Leishmania major Mice Pinna of ear Xylazine

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Variable analysis

independent variables

Dose of bacteria (low: 1.5x10^3 CFU/mL, high: 1.5x10^6 CFU/mL)
Presence or absence of L. major (1.5x10^6/mL)

dependent variables

Not explicitly mentioned

control variables

Volume of PBS (10 μL)
Route of administration (intradermal injection into the right ear pinna of mice)
Anesthesia (xylazine 10 mg/kg and ketamine 80 mg/kg)

controls

Positive control: L. major alone
Negative control: PBS

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