Mouse body weight was determined at baseline, before each drug administration and every week up to 8 weeks. In addition, mice were also examined daily for evaluation of general health including observation for signs of hair loss, piloerection, general gait weakness, condition of the hind paws and tail skin, and gastrointestinal disorders.
Ear cavity temperature was measured using an infrared thermometer (model IRT303HACCP, National Product, MD) at baseline and after weeks 1, 3, 6, and 8 prior to performing the behavioral tests. Core body temperature was measured using a rectal probe (Thermalert TH-5 and TCAT-1A Controller, Physitemp Instruments, Inc) at baseline and after weeks 1, 3, and 6 in two mice from each drug treatment group after brief anesthesia with isoflurane.
The nephrotoxicity of cisplatin and oxaliplatin was assessed by blood urea nitrogen (BUN) levels in samples collected at the end of the 3-week drug treatment. Based on normal mouse BUN values (8–33 mg/dl according to normal reference laboratory values from Research Animal Resources at the University of Minnesota-
Ear cavity temperature was measured using an infrared thermometer (model IRT303HACCP, National Product, MD) at baseline and after weeks 1, 3, 6, and 8 prior to performing the behavioral tests. Core body temperature was measured using a rectal probe (Thermalert TH-5 and TCAT-1A Controller, Physitemp Instruments, Inc) at baseline and after weeks 1, 3, and 6 in two mice from each drug treatment group after brief anesthesia with isoflurane.
The nephrotoxicity of cisplatin and oxaliplatin was assessed by blood urea nitrogen (BUN) levels in samples collected at the end of the 3-week drug treatment. Based on normal mouse BUN values (8–33 mg/dl according to normal reference laboratory values from Research Animal Resources at the University of Minnesota-
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