The protocol was approved by the Institutional Review Board. Informed consent was obtained from all participants prior to the study. Twenty healthy adults (twelve females and eight males) age 22–52 years (average 34 years; without diabetes or known vascular disease) were studied supine or prone in a 7T system (Achieva, Philips Medical Systems, Cleveland, OH). Spectra were acquired with a partial-volume quadrature transmit/receive coil customized to fit the shape of a human calf. Axial, coronal, and sagittal T2-weighted turbo spin echo images were initially acquired of the left calf muscle. Typical parameters were: field of view 180 × 180 mm, repetition time (TR) 1,500 ms, echo time (TE) 75 ms, turbo factor 16, and number of acquisitions (NA), 1. Single-voxel stimulated echo acquisition mode (STEAM) (typical parameters: voxel size 5 × 5 × 5 mm3 (∼0.1 ml), TR 2,000 ms, TE 20 ms, spectral BW of 4 kHz, number of points (NP) 4,096 and zero-filled to 8,192 prior to Fourier transform, NA 16, no water suppression) was used to acquire 1H spectra from tibial bone marrow and subcutaneous fat tissue. To correct individual resonances for relaxation effects, T1 and T2 were measured in seven of the subjects. T1 was measured using inversion-recovery, with nine inversion delay times in the range of 5 ms to 3,000 ms, with TR 7 s and TE 40 ms. T2 was measured by using ten TE values from 20 ms to 180 ms, with TR 8 s. Subjects were instructed to move slowly in the scan room. The entire scanning session was 60 min or less and it was well-tolerated by all subjects. All subjects were interviewed after the exam and again at 24 h after the exam. All subjects specifically denied dizziness, nausea, vertigo, headaches, or visual changes.