Optogenetic Activation of ChR2 Neurons
Corresponding Organization : University of Virginia
Other organizations : Tianjin Medical University, Tianjin Medical University Eye Hospital, Tianjin Medical University General Hospital
Variable analysis
- Intensity of LED light (~ 160 mW/mm^2) at the tip of the optic fiber
- Activation of ChR2-expressing neurons
- Optic fiber (0.2 mm core diameter) placed ~0.5 mm above the exposed SC
- Blue LED (470 nm, Doric Lenses) used for photostimulation
- The intensity of LED light was confirmed to be reliably effective in activating ChR2-expressing neurons (Shi et al., 2017)
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