Protocol detail

Plasma Biomarkers of Thrombosis and Inflammation

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Beta-thromboglobulin (βTG), prothrombin fragments 1+2 (F1+2), were measured by commercial enzyme-linked immunosorbent assay (ELISA) in plasma samples collected in CTAD tubes as previously described (31 EDTA plasma was used to measure histamine (ELISA kit Starfish) and C3a levels (Microvue Complement C3a Plus, Quidel) (29 (link)). Blood, collected in EDTA tubes containing 100μl of meclofenamate (10μg/ml, Sigma-Aldrich), was used for thromboxane (Thromboxane B2 EIA Kit).

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Burdorf L., Harris D., Dahi S., Zhang T., Ali F., Shah A., Thompson M., Braileanu G., Cheng X., Sievert E., Schwartz E., Sendil S., Parsell D.M., Redding E., Phelps C.J., Ayares D.L., Azimzadeh A.M, & Pierson RN 3.r.d. (2018). THROMBOXANE AND HISTAMINE MEDIATE PVR ELEVATION DURING XENOGENEIC PIG LUNG PERFUSION WITH HUMAN BLOOD. Xenotransplantation, 26(2), e12458.

Publication 2018

Microvue Complement C3a Plus meclofenamate

Beta thromboglobulin Blood Complement c3a Edta Enzyme linked immunosorbent assay Histamine Meclofenamate Plasma Prothrombin Starfish Thromboxane Thromboxane b2

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Beta-thromboglobulin (βTG)
Prothrombin fragments 1+2 (F1+2)
Histamine
C3a levels
Thromboxane

control variables

CTAD tubes (for βTG and F1+2 measurement)
EDTA tubes containing 100μl of meclofenamate (10μg/ml, Sigma-Aldrich) (for thromboxane measurement)
EDTA plasma (for histamine and C3a levels measurement)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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