Tethered Conformation Capture of Hypoxic Cells

For two replicates, 90 million cells were grown in normoxic (20% O₂) or in hypoxic (1% O₂) conditions (Ruskinn InVivo2 400 hypoxia incubator) for 8 h. Tethered conformation capture (TCC) was performed as described (3 (link)). Digestions were performed using MboI (New England Biolabs), DNA was sheared with Bioruptor Next-Gen (Diagenode) for 30–35 cycles, library preparations continued as described (3 (link)) and products were amplified for 15 cycles, size-selected (225–550 bp) from 10% TBE gels (Life Technologies) and sequenced using Illumina HiSeq 2000 at EMBL Genomics Core Facility (Heidelberg, Germany).